Molecular events involved in ochratoxin A induced mitochondrial pathway of apoptosis, modulation by Bcl‐2 family members
Identifieur interne : 000083 ( France/Analysis ); précédent : 000082; suivant : 000084Molecular events involved in ochratoxin A induced mitochondrial pathway of apoptosis, modulation by Bcl‐2 family members
Auteurs : Chayma Bouaziz [Tunisie] ; Ossama Sharaf El Dein [France] ; Cécile Martel [France] ; Emna El Golli [Tunisie] ; Salwa Abid-Essefi [Tunisie] ; Catherine Brenner [France] ; Christophe Lemaire [France] ; Hassen Bacha [Tunisie]Source :
- Environmental Toxicology [ 1520-4081 ] ; 2011-11.
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Abstract
In this study, we looked for the role of the mitochondrion in the cytotoxicity of ochratoxin A (OTA), which is one of the most abundant food‐contaminating mycotoxins in the world. In different human carcinoma cell lines, OTA triggered a mitochondria‐dependent apoptotic process, which is characterized by opening of the mitochondrial permeability transition pore (PTPC), loss of mitochondrial transmembrane potential (ΔΨm), increase in O2[chemp]– production, mitochondrial relocalization of Bax, release of cytochrome c, and caspase activation. However, studies performed on purified organelles suggested that OTA does not directly target the mitochondrion. In addition, we showed that mitochondrial alterations induced by this mycotoxin are favored by the proapoptotic protein Bax, but not Bak. These alterations are prevented by the antiapoptotic proteins, Bcl‐2 and to a lesser degree by Bcl‐XL. Taken together, these data indicate that although mitochondria, PTPC members and proteins of Bcl‐2 family play a pivotal role in OTA‐induced apoptosis, they do not constitute real targets to overcome its toxicity. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2011.
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DOI: 10.1002/tox.20581
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